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Christopher Hardin, Ph.D.

Professor HardinC@missouri.edu Visit Dr. Hardin's Lab Site

Research Interests

• The regulation and organization of glycolysis
• Metabolomics
• Diabetes and smooth muscle metabolism
• Lipid metabolism and lipotoxicity - mitochondrial dysfunction in diabetes, atherosclerosis, and cell phenotype transformation
• Magnetic resonance measures of cellular metabolism
• Smooth muscle physiology/pathophysiology
• Localized metabolism-function coupling
• Cytoarchitecture (caveolae, cytoskeleton) and metabolic organization
• Macromolecular organization and protein-protein interactions
• Energetic support of cell signaling

Background  Information

• B.S. from Cornell University
• Ph.D. in Physiology/Biophysics from the University of Cincinnati
• NIH funded postdoctoral training at the University of Washington
• Member of several professional societies including the Biophysical Society, American Physiological Society, American Heart Association, Metabolomics Society, and the International Society for Heart Research
• Joined Department in 1993
• 2005 Chancellor’s Award for Research and Creative Activities in the Biological Sciences
• 1999 Dorsett L. Spurgeon Distinguished Medical Research Award
• Served as an ad hoc reviewer for NIH; guest reviewer for 31 different journals; Editorial Board of American Journal of Physiology: Cell, International Advisory Board for Physiological Research
• Chair (2004) Gordon Research conference on Macromolecular Organization and Cell Function, Oxford UK ( http://www.grc.org/programs/2004/macromol.htm)
• Research currently funded by the National Institutes of Health
  

Selected  Publications

• Raikar, L.S., Vallejo, J., Lloyd. P.G. and Hardin, C.D. (2006). Overexpression of Caveolin-1 Results in Increased Plasma Membrane Targeting of Glycolytic Enzymes: The Structural Basis for a Membrane Associated Metabolic Compartment. Journal of Cellular Biochemistry (in press)
• Hardin, C.D. and Vallejo J. (2006). Caveolins in Vascular Smooth Muscle: Form Organizing Function Cardiovascular Research (in press)
• Vallejo, J. and Hardin, C.D. (2005) Expression of caveolin-1 in lymphocytes induces caveolae formation and recruitment of phosphofructokinase to the plasma membrane FASEB J. Apr;19(6):586-7. http://www.fasebj.org/cgi/doi/10.1096/fj.04-2380fje;
• Vallejo, J. and Hardin, C.D. (2004) Caveolin-1 Functions as a Scaffolding Protein for Phosphofructokinase in the Metabolic Organization of Vascular Smooth Muscle Biochemistry 43(51):16224-32.
• Vallejo, J, and Hardin C.D. (2004). Metabolic organization in vascular smooth muscle: distribution and localization of caveolin-1 and phosphofructokinase. American Journal of Physiology: Cell Physiology 286: C43-C54.
• Hardin, C.D., Kleiber, B.D., and Roberts, T.M. (2003). Mitochondrial oxidative substrate selection in porcine bladder smooth muscle. Journal of Urology 170: 2063-2066.
• Hardin, C.D., Lazzarino, G., Tavazzi, B, DiPierro, D, Roberts, T, Giardina, B, and Rovetto, M.J. (2001). Myocardial metabolism of exogenous FDP is consistent with transport by a dicarboxylate transporter. American Journal of Physiology. 281: H2654-2660.
• Allen, T.J. and Hardin, C.D. (2001). Oleate oxidation and mitochondrial substrate selection in vascular smooth muscle. Journal of Vascular Research 38(3): 276-287.
• Lloyd, P.G., and Hardin, C.D. (2001). Caveolae and carbohydrate metabolism in vascular smooth muscle. J. Cell Biochem 82: 399-408.
• Roberts, T.M., Sturek, M., Dixon, J., and Hardin, C.D. (2001). Alterations in the oxidative metabolic profile in vascular smooth muscle from atherogenic swine. Molecular and Cellular Biochemistry 217: 99-106.
• Lloyd, P.G., and Hardin, C.D. (2000). Sorting of metabolic pathway flux by the plasma membrane in cerebrovascular smooth muscle cells. American Journal of Physiology: Cell Physiology. 47: C803-811.
• Hardin, C.D., Lazzarino, G., Tavazzi, B, DiPierro, D, Roberts, T, Giardina, B, and Rovetto, M.J. (2001). Myocardial metabolism of exogenous FDP is consistent with transport by a dicarboxylate transporter. American Journal of Physiology. 281: H2654-2660.
• Hardin C.D. (2001). Making sense of oxygen sensing. Journal of Physiology 536: 1.
• Hardin, C.D., Allen, T.J., and Paul, R.J. (2001). Metabolism and energetics of vascular smooth muscle. In: Physiology and Pathophysiology of the Heart., 4th Edition, N. Sperelakis (ed)., Kluwer Academic Publishers, Norwell, MA. pp 571-595.
• Allen, T.J. and Hardin, C.D. (2001). Oleate oxidation and mitochondrial substrate selection in vascular smooth muscle. Journal of Vascular Research 38(3): 276-287.
• Lloyd, P.G., and Hardin, C.D. (2001). Caveolae and carbohydrate metabolism in vascular smooth muscle. J. Cell Biochem 82: 399-408.
• Roberts, T.M., Sturek, M., Dixon, J., and Hardin, C.D. (2001). Alterations in the
  oxidative metabolic profile in vascular smooth muscle from atherogenic swine. Molecular and Cellular Biochemistry 217: 99-106.
• Lloyd, P.G., and Hardin, C.D. (2000). Sorting of metabolic pathway flux by the plasma membrane in cerebrovascular smooth muscle cells. American Journal of Physiology: Cell Physiology. 47: C803-811.
• Allen, T.J. and Hardin, C.D. (2000). The influence of glycogen storage on vascular smooth muscle metabolism. American Journal of Physiology: Heart and Circulatory Physiology. 278: H1993-2002.
• Lloyd, P.G., and Hardin, C.D. (1999). The role of microtubules in the regulation of metabolism in isolated cerebral microvessels. American Journal of Physiology: Cell Physiology 277: C1250-1262.
• Lloyd, P.G., Hardin, C.D., and Sturek, M. (1999). Examining glucose transport in single vascular smooth muscle cells with a fluorescent glucose analog. Physiological Research 48: 401-410.
• Finder, D.R. and Hardin, C.D. (1999). Transport and metabolism of exogenous fumarate and 3-phosphoglycerate in vascular smooth muscle. Molecular and Cellular Biochemistry 195:113-121.
• Allen, T.M. and Hardin, C.D. (1998). The pattern of substrate utilization in vascular smooth muscle using 13C-isotopomer analysis of glutamate. American Journal of Physiology: 275(44): H2227-H2235.
• Hardin, C.D. and Finder, D.R. (1998). Glycolytic flux in permeabilized freshly isolated vascular smooth muscle cells. American Journal of Physiology: 274(43): C88-C96.
  

Methodolgy/Techniques

• Use of siRNAs to “knock down” scaffolding protein levels
• Use of overexpression of caveolin-1 in smooth muscle cells and lymphocytes
• Confocal microscopy
• Nuclear magnetic resonance spectroscopy
• Our plans include the use constructs for scaffolding proteins and glycolytic enzymes expressed with different fluorescent proteins (CFP, YFP) to perform FRET measures to better characterize the dynamic associations of these enzymes with structural proteins in cells.
• Metabolomics
  

See a recent CV from Dr. Hardin